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KMID : 1059520080520030273
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Journal of the Korean Chemical Society
2008 Volume.52 No. 3 p.273 ~ p.280
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Detection of Mycobacterium Tuberculosis by Loop-Mediated Isothermal Amplification Assay
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Ahn Young-Chang
Jang Won-Choeul
Nam Youn-Hyoung
Cho Min-Ho
Park Su-Min
Seo Jae-Won
Yoon Il-Kyu
Park Yong-Hyun
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Abstract
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Mycobacterium tuberculosis (MTB) remains a major worldwide public health problem. In recent years, the incidence of MTB has been rising. Rapid and reliable diagnosis of Mycobacterium tuberculosis is essential to initiate correct treatment, avoid severe complications, and prevent transmission. LAMP was used to develop a rapid and sensitive laboratory diagnostic system for the MTB. In this research, the loop-mediated isothermal amplification method (LAMP) that amplifies DNA with high specificity and rapidity at an isothermal condition was evaluated for rapid detection of MTB. Undiluted DNA (2.10 × 106 copy/mL), 10-1, 10-2, 10-3, 10-4, 10-5 and 10-6 (copy/mL) of MTB DNA were amplified by PCR and LAMP to determine the sensitivity of the assay. At results, the LAMP assay reported here has the advantages of rapid amplification, high sensitivity, and high specificity and will be useful for rapid and reliable clinical diagnosis of MTB in hospital clinical laboratory.
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KEYWORD
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FIP(Forward Inner Primer), BIP(Back Inner Primer), MTB(Mycobacterium Tuberculosis), PCR, LAMP(Loop Mediated Isothermal Amplification), FIP(Forward Inner Primer), BIP(Back Inner Primer)
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